Coding
chlorR-AB

Part:BBa_K349118:Design

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-21)

chlorR-AB (BioBytes 2.0)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 786


Design Notes

This part has built-in BsaI cutsites surrounding a P1004 chloramphenicol antibiotic resistance cassette. Digestion at these sites produces an A overhang at the 5' end and a B overhang at the 3' end consistent with the Biobytes 2.0 assembly method. NOTE: This part is in a pSB1A3 backbone because the pSB1C3 backbone already contains chloramphenicol resistance.

Source

pSB1A3

References